Worm Breeder's Gazette 14(3): 17 (June 1, 1996)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
box 45, Novosibirsk, 630107,USSR
It is well known that the royal jelly markedly prolongs the life span of honey bees. This substance is recommended by physicians for increasing of life quality. In this experiment royal jelly extract was used in the form of "Apilac" in dilutions 0,02% and 0,002%. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without royal jelly extract) during 6 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). Beginning from third day in the whole experiment was used the medium without this royal jelly extract. A number of progeny was calculated every day. This investigation was carried out in temperature +21C and in the darkness. The obtained results are presented in the following table. Control group Experimental Experimental group (0,02%) group (0,002%) n = 11 n = 11 n = 12 Mean +/- S.D. Mean +/- S.D. Mean +/- S.D Longevity (days): mean 19,4 +/- 1,2 18,0 +/- 1,7 21,8 +/- 1,2 maximal 25 27 26 minimal 10 9 14 Periods (days): prereproductive 2 2 2 reproductive 6,5 +/- 0,5 7,4 +/- 0,6 5,9 +/- 0,4 postreproductive 10,6 +/- 1,2 8,5 +/- 1,6 14,0 +/- 1,3 Fecundity: mean 158,8 +/- 6,6 93,9 +/- 9,9 128,9 +/- 3,4 maximal 197 179 153 minimal 115 60 111 Conclusion: If the extract from royal jelly was applied to C. elegans during prereproductive period of their life span, in above described conditions, it was not able to increase significantly their longevity as well as fecundity. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP50.