Worm Breeder's Gazette 14(2): 86 (February 1, 1996)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
|1||Institut Gustave Roussy, Villejuif, France|
|2||CNRS-CRBM, Montpellier, France|
The Hir protein family comprises HIR1 and HIR2, two proteins identified in yeast as histone transcriptional regulators, and the product of the HIRA gene cloned from the chromosome 22 region critical for the DiGeorge syndrome, a human haploinsufficiency disease affecting derivatives of the third and fourth pharyngeal pouches (Lamour et al., Hum. Mol. Genet. 1995, 5: 731-739). These WD repeat-containing proteins appear to play a role in nuclear architecture as part of multiprotein complexes. We have identified a family member through screening of the C. elegans database. The corresponding gene, K10D2.1, maps to chromosome III, between the cloned genes ced-4 and clk-1. Using primers from the 3' end of the yk22d7 cDNA (kindly provided by Yuji Kohara), we have shown by Southern blotting that a unique gene in C. elegans hybridizes to our .2 kb probe and have designed a PCR assay to test for the presence of K10D2.1 in the deficiencies of the area, kindly provided by David Baillie. To our surprise, the gene is present in the three deficiencies sDfl30, sDfl21 and sDf134, indicating that it maps left of their left limits. We propose below a revised map in which all the genetic and molecular data is consistently taken into account. To identify a strain with a mutation in the C. elegans HIR gene, the K10D2 cosmid will be tested for its ability to rescue selected mutants. (A figure accompanies the original article.)