Worm Breeder's Gazette 14(2): 34 (February 1, 1996)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Tokyo Metropolitan Institute of Gerontology Tokyo 173 Japan, hondas@center.tmig.or.jp
Dauer larva can survive for long periods in harsh environments. Some
of the daf genes that control dauer formation are expected to be
involved in the acquisition of resistance to severe stress and extention
of life span. We are interested in oxidative stress because oxidative
stress is postulated to be one of the causative agents in aging. In order
to clarify the mechanism of acquisition of stress resistance and
extension of life span, the susceptibility to oxidative stress has been
measured in various daf mutants and their double and triple mutants. In
ts mutants, measurements were done at non-permissive temperatures
after the L4 stage.
daf-1 mutant, daf-3, daf-4, daf-5, daf-6, daf-7, daf-8, daf-10,
daf-12, daf-13, daf-14, daf-16, daf-18, daf-19 and daf-22 are similar
to wild type N2 with respect to susceptibility to oxidative stress.
daf-2(e1370) and daf-2(e1286) are more resistant to oxidative
stress than N2. On the other hand daf-2(m41) and daf-2(e1368) were
similar to N2.
daf-23(m333) are more resistant to oxidative stress than N2.
daf-2(e1370); daf-3(e1376) double mutant and daf-5(e1386); daf-2
(e1370) are similar to daf-2(e1370).
daf-16(m26); daf-2(e1370) and daf-18(e1375); daf-2(e1370) are
similar to N2.
daf-2(e1370); daf-12(m20) is somewhat more resistant than daf-2
(e1370).
daf-16(m26); daf-2(e1370); daf-12(m20) triple mutant is similar
to N2.
daf-16(m26); daf-23(m333) is similar to N2. daf-18(e1375); daf-23
(m333) is intermediate between daf-18 and daf-23 .
We have measured the adult life span of long-life mutants: daf-2
and daf-2; daf-12 under hyperoxia. N2 life span under hyperoxia was
about 70% of that under an air atmosphere. By contrast, the life span
of daf-2(e1370) under hyperoxia was about 90% of that under air
atmosphere, and the life span of daf-2(e1370); daf-12(m20) was
almost the same as that under air atmosphere, indicating correlation
between oxidative-stress resistance and longevity.
These results indicate that the characteristic genetic pathway
involved in acquiring oxidative-stress tolerance is similar to that
involved in determining longevity (Larsen et al., '95).
daf-12
|
daf-2 - daf-18 - daf-23 - daf-16 - (oxidative-stress resistance)
References: S.Honda et al., Mech. Age. Devel. 63 235 '92, J. Gerontol. 48
B57 '93, C. Kenyon et al., Nature 366 461 '94, P. L. Larsen et al.,
Genetics
139 1567 '95
Acknowledgement: We thank Dr. Don L. Riddle, MRC and CGC for strains.