Worm Breeder's Gazette 14(2): 22 (February 1, 1996)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
CNRS-CRBM and Physique Math\'ematique, Montpellier, France.
A rapidly expanding portion of the sequence of the wild type is now available. So, many laboratories face the problem of sequencing their pet alleles, looking for mutations. Ace.mbly is a new graphic software which should help in this task. It is built over acedb and runs on any Unix platform. The system is self contained and hopefully easy to use. If you are sequencing on an ABI machine, then, following a straightforward protocol, ace.mbly will import the traces, clip the vectors, clip the end of the sequences as a function of the quality of the traces and align your sequences together and/or with the wild type. You can then visualize all discrepancies and access the traces to fix the rather frequent errors of the ABI base call or to confirm that you found a genuine mutation. The system was written for large scale sequencing. It can assemble a complete cosmid in a few minutes and can be used as an alternative to (or in conjunction with) PHRAP and GAP. But we believe that ace.mbly is also very useful in a small lab without computing expertise and hope to receive your comments and wish lists. Ace.mbly is freely available by ftp from //ncbi.nlm.nih.gov/repository/acedb/acembly