Worm Breeder's Gazette 14(2): 22 (February 1, 1996)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

ACE.MBLY, a graphic program for wild type and mutant sequence assembly

Danielle et Jean Thierry-Mieg , Ulrich Sauvage

CNRS-CRBM and Physique Math\'ematique, Montpellier, France.

A rapidly expanding portion of the sequence of the wild type is now
available. So, many laboratories face the problem of sequencing their
pet alleles, looking for mutations.

Ace.mbly is a new graphic software which should help in this task.
It is built over acedb and runs on any Unix platform. The system is
self contained and hopefully easy to use. If you are sequencing on
an ABI machine, then, following a straightforward protocol, ace.mbly
will import the traces, clip the vectors, clip the end of the
sequences as a function of the quality of the traces and align your
sequences together and/or with the wild type. You can then visualize
all discrepancies and access the traces to fix the rather frequent
errors of the ABI base call or to confirm that you found a genuine

The system was written for large scale sequencing. It can assemble a
complete cosmid in a few minutes and can be used as an alternative
to (or in conjunction with) PHRAP and GAP. But we believe that ace.mbly is
also very useful in a small lab without computing expertise and hope to
receive your comments and wish lists.

 Ace.mbly is freely available by ftp from