Worm Breeder's Gazette 14(1): 106 (October 1, 1995)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
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C. elegans (1 - 2 days old) were collected from separate stock, frozen and after 12 hours or more time, thawed, filtrated and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3 - 5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without young worms extract) during 12 hours then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). The medium with extract from young worms was used in experimental group beginning from third day, that is in reproductive and postreproductive periods of nematode lie span. Number of progeny was calculated every day. This investigation was carried out in temperature +21 C and in the dark- ness. The obtained results are presented in the following table. Control group Experimental group n=11 P n=10 Mean+/-S.D. Mean+/-S.D. Longevity: mean 19,2 +/- 1,9 >0,05 24,2 +/- 2,4 maximal 30 35 minimal 11 11 Period: prereproductive 3,4 +/- 0,3 >0,05 2,9 +/- 0,1 reproductive 10,2 +/- 0,9 <0,05 14,0 +/- 1,3 postreproductive 4,8 +/- 1,2 >0,05 6,9 +/- 1,7 Fecundity: mean 146,8 +/- 10,3 <0,02 199,4 +/-16,2 maximal 182 330 minimal 75 113 Conclusion: If the extract from young worms was applied to C. elegans during reproductive and postreproductive period of their life span, then it was able to increase their mean fecundity (by 35,8 per cent) as well as length of reproductive period (by 37,3 per cent). The mean longevity in experimental group was slightly increased too (but not significantly). Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E.coli OP50.