Worm Breeder's Gazette 14(1): 105 (October 1, 1995)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
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C. elegans (1-2 days old) were collected from separate stock, frozen and after 12 hours or more time thawed, filtrated and mixed with S medium containing E. coli (1:1), this mixture was used as a medium for worms in experimental group. The medium for control group was prepared by mixing S medium containing E. coli with S medium without E. coli (1:1). Three adult animals (3-5 days old) were kept in microtitre wells containing 0,75 ml of liquid medium (with E. coli and with or without young worms extract) during 12 hours, then they were discarded and newborn larvae were transferred in next wells every day (one worm in one well). Number of progeny was calculated every day too. This experiment was carried out in temperature 21 C and in the darkness. The obtained results are presented in the following table. Control group Experimental group n = 12 P n = 11 Mean +/- S.D. Mean +/- S.D. Longevity: mean 24,5 +/- 1,9 <0,001 13,6 +/- 1,5 maximal 36 21 minimal 13 8 Period: prereproductive 3,9 +/- 0,08 <0,05 4,4 +/- 0,2 reproductive 9,9 +/- 0,09 <0,01 6,4 +/- 1,2 postreproductive 9,9 +/- 1,8 <0,001 2,8 +/- 0,7 Fecundity: mean 123,9 +/- 12,7 <O,001 33,6 +/- 7,6 maximal 219 69 minimal 13 o Conclusion: if the extract from young worms was applied to C. elegans during whole life span then it was able to decrease longevity of nematodes, by other words such an extract appeared as toxic, in indicated above conditions. This extract decreased the fecundity too. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (Bristol, N2) and E. coli OP 50.