Worm Breeder's Gazette 14(1): 103 (October 1, 1995)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
box 45, Novosibirsk, 630107, USSR
The purpose of the present experiment is to investigate, how low temperatures in the early stages of development can influence fecundity, length of prereproductive period, length of reproductive period, length of postreproductive period ad mean life span in the nematode C. elegans (Bristol, N2, wild type). To microtitre wells were added 0,75 ml of liquid medium with E. coli (OP 50) and 3 adult nematodes. Animals were kept during 12 hours in the room temperature, then adult nematodes were discarded and wells were stored in different temperatures during 2 days, then they were transferred in room temperature. Newly appeared worms were transferred in next wells, every well contains a single nematode. In every following day these worms were transferred in next wells. All the experiments were done in the darkness. Tempe- Number Length of Mean rature of life C progeny prerepro- reproduc- postrep- span ductive tive roductive days period period period n days days days 21,3 79,8+/-5,4 2,2+/-0,1 8,3+/-0,4 12,8+/-1,1 24,4+/-1,1 26 9,0 77,0+/-5,5 3,3+/-0,1 7,7+/-0,6 11,1+/-1,3 22,8+/-1,5 25 63 67,8+/-3,4 3,8+/-0,1 7,0+/-0,4 11,7+/-1,5 23,2+/-1,5 23 2,0 68,5+/-5,0 4,3+/-0,2 7,1+/-0,4 8,6+/-1,2 21,1+/-1,1 20 Then, lowering of ambient temperatures during early development of nematode C. elegans resulted in decreasing of progeny. There is a decrease in the mean life span as temperature during larval development decreased, as well as the length of reproductive and the length of postreproductive periods. Then, the length of prereproductive period is increased by diminishing of the temperature of early development. These results demonstrate, that the higher is the temperature, the earlier nematodes started reproduction. These results do not support the widely accepted point of view, that lowering of temperature increases life span of different animals. There exist optimal life conditions for species, in which life span of this species is maximal. But the problem arises: what must we do in order to obtain the marked increase of longevity and, probably, immortality? Undoubtedly, it appeared reasonable to seek a new unusual experimental approaches. Acknowledment: he author wishes to express his thanks to CGC for providing C. elegans and E. coli.