Worm Breeder's Gazette 13(5): 84 (February 1, 1995)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
|1||Research and Development Headquarters, Biological Science Research Center, Lion Corporation, Tajima, Odawara, Kanagawa, 100|
|2||Department of Molecular Biology, Tokai University School of Medicine, Isehara, Kanagawa 259-1 1, Japan|
Mutation in mev-1 render animals hypersensitive to high oxygen concentration (1). The life span of this mutant is shorter than that of wild type under atmospheric conditions and various depending upon the oxygen concentration (2). To further our understanding of mev-1, we. measured the level of peroxylipid and oxidized protein as assessed by fluorescent materials and carbonyl residues, respectively.These are good markers of aging, as it accumulated with increasing aging. Fluorescent materials in methanol/water extracts of both wild type and mev -1 animals accumulated with increasing age. The fluorescent material in mev-1 accumulated more than in the wild type. The amount of the extracts of mev-1 on day 10 was approximately two times that observed in the wild type on the same day (3). We also found that the mev-1 mutant accumuiates more carbonyl residues at a greater rate than dose wild type. When incubated under 90percent oxygen, the fluorescent materials in the mev-1, but not wild-type animals accumulated more rapidly compared with when incubated under atmospheric condition. Conversely, the materials did not accumulated in either wild type or mev-1 animals under 2percent oxygen3. The rate of accumulation of fluorescent material was influenced by oxygen.The activity of superoxide dismutase was about half the wild-type level(1). These observation suggests that the mev-1 may be a mutant of premature aging and oxygen may be a determinant of aging in C. elegans. 1. N. Ishii et al, Mutation Research, 237, 16*171 2. S. Honda et al, J. Geront.48, B*7-B61 (1993) 3. H. Hosokawa et al, Mech. Ageing Develop. 74, 161- 1 70 (1994)