Worm Breeder's Gazette 13(5): 68 (February 1, 1995)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
University of Colorado - Boulder, Campus Box 347, Boulder, CO 80309 The ct344 mutation, isolated by Lois Edgar, results in nonviable embryos, some of which hatch and die as L1's with disorganized posteriors. In this way ct344 resembles other nob mutations being characterized in this laboratory (1) and as a result, it was previously referred to as nob-5(ct344). It is unique, however, in that it displays a partial maternal effect. Heterozygous hermaphrodites give rise to phenotypically wild-type homozygous mutant progeny, which produce 100% Nob animals and dead eggs, unless mated with a wild-type male. This mutation was mapped to LGV(L) near unc-62. Complementation tests showed that it fails to complement either the Unc phenotype resulting from unc-62(e644) or the non-maternal effect embryonic lethal phenotype resulting from unc-62(s472). Examination of e644 homozygous mutant animals reveals that about 19% of their progeny display the Nob phenotype, though neither this nor the Unc phenotype show the maternal effect described for ct344. Results of dosage experiments shown in the table below suggest that s472 is a nearly complete loss-of-function mutation, and that ct344 is completely recessive. In addition, e644/s472 trans-heterozygotes display a maternal effect that is not seen in the homozygous e644 mutant animals. Further dosage experiments and phenotypic characterization of the embryonic lethal phenotypes resulting from ct344 and s472 are in progress. F1 homozygotes F2 homozygotes or trans-heterozygotes or trans-heterozygotes Genotype %Nob %dead eggs %viable %Nob %dead eggs %viable --------- ----- ---------- ------- ---- ---------- ------- e644/e644 19 5 76 19 5 76 ct344/ct344 12 nd 87 42 57 <1 e644/sDf26 8 51 40 No F1 animals survive to produce progeny ct344/sDf26 9 56 33 No F1 animals survive to produce progeny e644/s472 25 30 45 6 82 11 ct344/s472 23 30 47 No F1 animals survive to produce progeny ct344/ct344/yDp1 <2 <2 >95 * * These percentages are estimates. Good brood counts have not yet been performed. We used the e644 mutation to identify the region of the physical map that includes unc-62. Using polymorphisms between the Bristol and Bergerac strains as reference points, we mapped this mutation to the region between stP3 and another polymorphism associated with the cosmid RW#L63. Two deficiency break points fall in this region: the right end of sDf50 and the right end of sDf27. PCR mapping experiments of these deficiencies performed by A. Scouras and M.F. Wakarchuk in David Baillie's laboratory further limit the candidate physical region. These compiled results leave two candidate cosmids and the surrounding cosmid gaps. The candidate cosmids are being injected into the ct344 and s472 mutant strains. (1) Edgar, L.G., N. Wolfe, S.H. Carr, and W.B. Wood. "The Nog genes and pal-1 are involved in embryonic posterior morphogenesis." 1993 Worm Meeting Abstracts: p.10.