Worm Breeder's Gazette 13(4): 96 (October 1, 1994)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

New Deficiencies on LGIII.

Heinke Schnabel, Ralf Schnabel

Max-Planck-Institut fur Biochemie, D-82152 Martinsried, Fed. Rep. of Germany

  In an attempt to generate new deficiencies on LGIII (which we need to analyze
the maternal-effect lethals that we have generated) we mutagenized unc-32 ( e189 )/
qC1 worms with 7500R. A total of 5186 F1 animals were picked to single plates and
their progeny analyzed for the absence of Unc worms, indicating a lethal mutation
linked to unc-32 .904 had to be discarded for various reasons, 923 were sterile or
produced only dead eggs. Of the remaining 3359, 21 stable lethals linked to unc-32
were obtained after outcrossing. These lethals were then testcrossed with several
markers in order to find putative deficiencies. In this manner, we obtained 4
deficiencies which in addition to being lethal fail to complement mutations in the
following genes: (see figure)