Worm Breeder's Gazette 13(4): 96 (October 1, 1994)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Max-Planck-Institut fur Biochemie, D-82152 Martinsried, Fed. Rep. of Germany
In an attempt to generate new deficiencies on LGIII (which we need to analyze the maternal-effect lethals that we have generated) we mutagenized unc-32 ( e189 )/ qC1 worms with 7500R. A total of 5186 F1 animals were picked to single plates and their progeny analyzed for the absence of Unc worms, indicating a lethal mutation linked to unc-32 .904 had to be discarded for various reasons, 923 were sterile or produced only dead eggs. Of the remaining 3359, 21 stable lethals linked to unc-32 were obtained after outcrossing. These lethals were then testcrossed with several markers in order to find putative deficiencies. In this manner, we obtained 4 deficiencies which in addition to being lethal fail to complement mutations in the following genes: (see figure)