Worm Breeder's Gazette 13(4): 95 (October 1, 1994)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Institute of Molecular Biology and Biochemistry, Simon
Fraser University, Burnaby, B.C. Canada, V5A 1S6.
We have isolated 109 EMS induced recessive lethal
mutations linked to dpy-17(e364) and balanced by the the
free duplication sDp3. These mutations are on the left
arm of LGIII in a region sequenced by the C. elegans
Genome Sequencing Project. To date, 101 of these
mutations define 88 previously unidentified essential
genes. To assist in the mapping of these genes, we have
used either gamma or ultra-violet irradiation to generate
a set of putative deficiencies. These were isolated as 38
lethal mutations linked to dpy-17. To date,
complementation tests with the set of EMS induced lethals
have identified 4 deficiencies. As usual, the lethals
placed on the line of the map have been mapped to the
deficiencies. Those positioned above the line are
presently being complementation tested with the
deficiencies and have only been positioned by 3 factor
mapping. We are using the new genetic markers to
correlate the physical and genetic maps (see D. Collins,
et al. Correlating the Physical and Genetic Maps on
Chromosome III (Left) - A First Step.) The
characterization of the new balancer, sDp3, used in this
work was funded by the National Institute of Health (NIH).
The strains generated by this project are available to the
C. elegans genetics community and can be requested by e-
mailing: hstewart@darwin.mbb.sfu.ca
let-774
let-756
let-745
dpy-17 let-721
let-789 let-754 let-713
let-734 let-777 let-775 let-722 |
let-749 let-778 let-783 let-744 |
let-733 let-748 | let-753 let-723 |
let-732 mel-31 | let-705 sma-3 | |
| let-707 | let-708| | lon-1 | | | unc-32
___|_____|_________|___|______|__|______|______|_____|______|___
__|__
sDf121 sDf128
|___________________________________________| _ ______ _
| sDf12 | sDf125
_ _ ______________ _ _ |___|
| |
sDp3 |
__________________________________________________________________ |
__________________________________________________________________ |
|
Not all mutations are shown.