Worm Breeder's Gazette 13(4): 95 (October 1, 1994)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

A New Set of Deficiencies and 88 New Essential Genes Have Been Identified on LGIII(left) in Caenorhabditis elegans.,

H. Stewart, D. Collins and D. Baillie,

Figure 1

Institute of Molecular Biology and Biochemistry, Simon 
Fraser University, Burnaby, B.C. Canada, V5A 1S6.

We have isolated 109 EMS induced recessive lethal 
mutations linked to dpy-17(e364) and balanced by the the 
free duplication sDp3.  These mutations are on the left 
arm of LGIII in a region sequenced by the C. elegans 
Genome Sequencing Project.  To date, 101 of these 
mutations define 88 previously unidentified essential 
genes.  To assist in the mapping of these genes, we have 
used either gamma or ultra-violet irradiation to generate 
a set of putative deficiencies.  These were isolated as 38 
lethal mutations linked to dpy-17.  To date, 
complementation tests with the set of EMS induced lethals 
have identified 4 deficiencies.  As usual, the lethals 
placed on the line of the map have been mapped to the 
deficiencies. Those positioned above the line are 
presently being complementation tested with the 
deficiencies and have only been positioned by 3 factor 
mapping.  We are using the new genetic markers to 
correlate the physical and genetic maps (see D. Collins, 
et al. Correlating the Physical and Genetic Maps on 
Chromosome III (Left) - A First Step.)   The 
characterization of the new balancer, sDp3, used in this 
work was funded by the National Institute of Health (NIH).  
The strains generated by this project are available to the 
C. elegans genetics community and can be requested by e-
mailing:  hstewart@darwin.mbb.sfu.ca

                 dpy-17                                   let-721
                 let-789     let-754                      let-713
  let-734        let-777     let-775               let-722  |
  let-749        let-778     let-783               let-744  |
  let-733        let-748      | let-753            let-723  |
  let-732         mel-31      | let-705       sma-3  |      |
   |     let-707   |   let-708|  |     lon-1   |     |      |  unc-32

                 sDf121                            sDf128
|___________________________________________|    _ ______ _   
|                       sDf12               |                sDf125
                 _ _ ______________ _ _                       |___|
                                                              |   |
                     sDp3                                          |
__________________________________________________________________ |
__________________________________________________________________ |

 Not all mutations are shown.

Figure 1