Worm Breeder's Gazette 13(4): 95 (October 1, 1994)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Institute of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, B.C. Canada, V5A 1S6. We have isolated 109 EMS induced recessive lethal mutations linked to dpy-17(e364) and balanced by the the free duplication sDp3. These mutations are on the left arm of LGIII in a region sequenced by the C. elegans Genome Sequencing Project. To date, 101 of these mutations define 88 previously unidentified essential genes. To assist in the mapping of these genes, we have used either gamma or ultra-violet irradiation to generate a set of putative deficiencies. These were isolated as 38 lethal mutations linked to dpy-17. To date, complementation tests with the set of EMS induced lethals have identified 4 deficiencies. As usual, the lethals placed on the line of the map have been mapped to the deficiencies. Those positioned above the line are presently being complementation tested with the deficiencies and have only been positioned by 3 factor mapping. We are using the new genetic markers to correlate the physical and genetic maps (see D. Collins, et al. Correlating the Physical and Genetic Maps on Chromosome III (Left) - A First Step.) The characterization of the new balancer, sDp3, used in this work was funded by the National Institute of Health (NIH). The strains generated by this project are available to the C. elegans genetics community and can be requested by e- mailing: firstname.lastname@example.org let-774 let-756 let-745 dpy-17 let-721 let-789 let-754 let-713 let-734 let-777 let-775 let-722 | let-749 let-778 let-783 let-744 | let-733 let-748 | let-753 let-723 | let-732 mel-31 | let-705 sma-3 | | | let-707 | let-708| | lon-1 | | | unc-32 ___|_____|_________|___|______|__|______|______|_____|______|___ __|__ sDf121 sDf128 |___________________________________________| _ ______ _ | sDf12 | sDf125 _ _ ______________ _ _ |___| | | sDp3 | __________________________________________________________________ | __________________________________________________________________ | | Not all mutations are shown.