Worm Breeder's Gazette 13(4): 91 (October 1, 1994)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

ceh-24, a New NK Class Homeodomain Gene.

Brian Harfe1, Pete Okkema2, Andy Fire2

1 Biology Graduate Program, The Johns Hopkins University
2 Carnegie Institution of Washington, Department of Embryology

Figure 1

  The NK class of homeodomain proteins are a set of regulatory factors with a broad
evolutionary distribution and a variety of expression patterns. Analysis of pharyngeal
myosin regulation led to the identification of a member of this family, ceh-22 ,which
appears to mediate aspects of pharyngeal gene expression (Okkema and Fire,
Development 120, 2175). To determine the extent of the NK homeodomain family in C.
elegans we have used a degenerate primer based PCR strategy. Among several
candidates from the initial PCR, we have so far characterized one gene, denoted ceh-24
.The CEH-24 homeodomain falls into the NK class, but is closer to diverse members of
the NK family seen in other species than to CEH-22 .In addition to the homeodomain,
CEH-24 contains an acid activation domain C-terminal to the homeodomain. ceh-24
maps (thanks to Alan Coulson) to chromosome V just left of him-5 .(see figure)
  Kim and Nirenberg, PNAS 86:7716
  Price et al., Neuron 8:241
  Lints et al., Development 119:419
  Komuro and Izumo, PNAS 90: 8145
  Saha et al., Development 118: 193
  Nardelli-Haefliger and Shankland, Development 118: 877
  Guazzi et al., EMBO J. 9: 3631
  Garcia-Fernandez et al., PNAS 88: 7338
  Oliver et al., Gene 121: 337

Figure 1