Worm Breeder's Gazette 13(4): 91 (October 1, 1994)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
|1||Biology Graduate Program, The Johns Hopkins University|
|2||Carnegie Institution of Washington, Department of Embryology|
The NK class of homeodomain proteins are a set of regulatory factors with a broad evolutionary distribution and a variety of expression patterns. Analysis of pharyngeal myosin regulation led to the identification of a member of this family, ceh-22 ,which appears to mediate aspects of pharyngeal gene expression (Okkema and Fire, Development 120, 2175). To determine the extent of the NK homeodomain family in C. elegans we have used a degenerate primer based PCR strategy. Among several candidates from the initial PCR, we have so far characterized one gene, denoted ceh-24 .The CEH-24 homeodomain falls into the NK class, but is closer to diverse members of the NK family seen in other species than to CEH-22 .In addition to the homeodomain, CEH-24 contains an acid activation domain C-terminal to the homeodomain. ceh-24 maps (thanks to Alan Coulson) to chromosome V just left of him-5 .(see figure) Kim and Nirenberg, PNAS 86:7716 Price et al., Neuron 8:241 Lints et al., Development 119:419 Komuro and Izumo, PNAS 90: 8145 Saha et al., Development 118: 193 Nardelli-Haefliger and Shankland, Development 118: 877 Guazzi et al., EMBO J. 9: 3631 Garcia-Fernandez et al., PNAS 88: 7338 Oliver et al., Gene 121: 337