Worm Breeder's Gazette 13(4): 75 (October 1, 1994)
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Molecular Biology, Department of Biology, Faculty of Science, Okayama University, Okayama Japan 700 c5191 8@ jpnkudpc
Ryr-1 ;The worm has the ryanodine receptor gene (vol. 12 (3) p.51, Vol. 13 (2) p.58, Sakube et al.) and the functional protein could be the gene product (Vol. 12 (4) p.69 Maryon et al.). We have completed the structure of the gene on the cosmid clone M04C11 that spans 30 kb and encodes 46 exons. The gene locates between CeLip-1 and odc-1 LGV. To know the place of expressed gene, promoter- ryr-1 /lacZfusion plasmids were injected into worms and were analyzed the activity of, 337-galactosidase. At the moment, the ryr-1 gene was expressed in many tissues under the control of 5'UTS. Fusion plasmids having 4.8 k and 2.7 kb of 5'UTS were expressed in muscles. 1.5 kb fragment was expressed in intestine and nerve. Fragments having 1.3 kb and 0.8 kb were expressed in muscles or intestine and nerve cells. At the moment we have not a conclusive result. We also isolated cDNA clones from a library and products of RT-PCR and constructed expression plasmids for antibody isolation.