Worm Breeder's Gazette 13(4): 75 (October 1, 1994)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Studies on the Ryanodine Receptor Gene, ryr-1, kra-1 and unc-68 Genes in the LG V.

Yasuji Sakube, Tatsuji Ikeda, Hiroaki Kagawa

Molecular Biology, Department of Biology, Faculty of Science, Okayama University, Okayama Japan 700 c5191 8@ jpnkudpc

  Ryr-1 ;The worm has the ryanodine receptor gene (vol. 12 (3) p.51, Vol. 13 (2)
p.58, Sakube et al.) and the functional protein could be the gene product (Vol. 12 (4)
p.69 Maryon et al.). We have completed the structure of the gene on the cosmid clone
M04C11 that spans 30 kb and encodes 46 exons. The gene locates between CeLip-1 and
odc-1 LGV. To know the place of expressed gene, promoter- ryr-1 /lacZfusion
plasmids were injected into worms and were analyzed the activity of, 337-galactosidase.
At the moment, the ryr-1 gene was expressed in many tissues under the control of
5'UTS. Fusion plasmids having 4.8 k and 2.7 kb of 5'UTS were expressed in muscles.
1.5 kb fragment was expressed in intestine and nerve. Fragments having 1.3 kb and
0.8 kb were expressed in muscles or intestine and nerve cells. At the moment we have
not a conclusive result. We also isolated cDNA clones from a library and products of
RT-PCR and constructed expression plasmids for antibody isolation.