Worm Breeder's Gazette 13(4): 32 (October 1, 1994)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
|1||Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461.|
|2||Department of Biology, New York University, Rm 1009 Main Bldg., Washington Square, New York, NY 10003|
In the past, non-C. elegans species have been maintained without any strain designation, or sometimes with a two-letter postscript to indicate the site of collection. As the study of evolution of nematodes begins, and more and more laboratories work with increasing numbers of new species, this system is becoming burdensome. For example, there is no way to indicate multiple isolations at a single site. Worms originating from non-C. elegans laboratories tend to have no strain designation at all. There is a tendency not to report a strain name when a non-C. elegans species is mailed. Hence, independent isolates of species are being treated as equivalent, the information about their independent origins being lost. When worms are frozen in various laboratories, laboratory strain names, necessary for databases, are being assigned, with the result that equivalent populations of worms can acquire several different laboratory strain names. In view of this growing problem, we have developed the following recommendation for the uniform assignment of strain names to non-C. elegans species. We have reviewed these suggestions with Scott Baird, Lynn Carta, Bob Herman, Jonathan Hodgkin, and Patrick Phillips, and they are in agreement. We therefore suggest that the following practices be adopted: l. The old strain naming system, in which letters were placed after the species name to indicate the site of collection, is dropped. 2. Every population of worms has a single strain name in standard C. elegans format, assigned by a laboratory with a two-letter designation registered with the Caenorhabditis Genetics Center. The strain name is reported along with the species name, in the following format: Rhabditis any species (EM0000). 3. The strain name is assigned by the collecting laboratory. The collecting laboratory is the first laboratory with a registered strain designation that receives the population of worms. The worms might be collected from the wild, or they might be received from a laboratory without a registered strain designation. The strain name does not change as the worms are sent from laboratory to laboratory. 4. The collecting laboratory is responsible for maintaining information regarding the origin of the strain. Each population founded from a separate wild isolation receives a new strain name (experience with C. elegans wild isolates indicates these can differ significantly, eg with respect to transposon distribution). For a hermaphroditic species, the "founding population" can be a single worm. However, for male-female species, it is at least two worms, and could be many more, as there may be a problem with inbreeding depression if the founding population is too small. Therefore a strain designation does not imply uniform genetic homozygosity.