Worm Breeder's Gazette 13(4): 10 (October 1, 1994)
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.
We present here another progress report for the C. elegans genome sequencing project. A large region of chromosome III is now essentially complete, although some gaps remain where cosmids were not available (Figure 1). While we are rescuing these regions from YACs and lambda clones, we will press ahead with cosmid-rich regions of other chromosomes. Since June, we have made considerable progress on much of the central region of chromosome II. Significant protein similarities (blastx score >80) are presented in Table 1 for this region (all cosmids for which shotgun sequencing has been completed). For each genomic locus, the highest blastx score and a brief identifier are listed. Although the cosmids which contain database hits may not be completely sequenced, the Consortium will make preliminary sequence data available to the community with the caveat that it is preliminary and may still contain errors. Finished cosmid sequences are now available by anonymous ftp at: ftp.sanger.ac.uk (directory: pub/database/C.elegans_sequences). In addition to progress on chromosomes II and III, shotgun sequencing has begun on chromosome X. Beginning at kin-11 ,the St. louis group is going left (from cosmid C23F12 )and the Cambridge group is going right. Since there is good cosmid coverage, we intend to sequence nearly all of chromosome X before moving on the next chromosome. For information on homologies, please contact LaDeana Hillier (lhillier(@watson.wustl.edu) or Richard Durbin (email@example.com). For information on sequencing plans or estimated completion times, please contact Richard Wilson (firstname.lastname@example.org) or Alan Coulson (email@example.com). All requests for cosmid clones should be addressed to Alan Coulson.