Worm Breeder's Gazette 13(3): 11 (June 1, 1994)
These abstracts should not be cited in bibliographies. Material
contained herein should be treated as personal communication and
should be cited as such only with the consent of the author.
The C. elegans genome sequencing project. A progress report.
The C. elegans Genome Consortium, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.
We present here another progress report for the C. elegans genome
sequencing project. A detailed description of 2.181 Mb of finished,
contiguous sequence near the center of chromosome III has recently been
published (1). Homology data for several additional cosmids was presented
in the last issue of the Gazette (2). Since January, we have made
considerable progress on much of the remainder of chromosome III (Figure
1). Significant protein similarities (blastx score >80) for cosmids
completely or nearly finished since the last report are presented in
Table 1. For each genomic locus, the highest blastx score and a brief
identifier are listed. Although the cosmids which contain database hits
may not be completely sequenced, the Consortium will make preliminary
sequence data available to the community with the caveat that it is
preliminary and may still contain errors. Finished cosmid sequences are
now available by anonymous ftp at:
ftp.sanger.ac.uk (directory: pub/C.elegans_sequences).
In addition to chromosome III, shotgun sequencing is well along on
chromosome II. The St. Louis group is going left from cosmid C06A8 to
cosmid W06A11 ,and the Cambridge group is going right from cosmid
T05A6 to near the vmp-1 locus. Once
the gene-rich regions of chromosome II have been sequenced, the
Consortium will move to chromosome X. For information on homologies,
please contact LaDeana Hillier (firstname.lastname@example.org) or Richard
Durbin (email@example.com). For information on sequencing plans or
estimated completion times, please contact Richard Wilson
(firstname.lastname@example.org) or Alan Coulson (email@example.com). All
requests for cosmid clones should be addressed to Alan Coulson.
1) R. Wilson et al. Nature 368, 32-38 (1994).
2) The CeGC. WBG 13,14-19 (Feb. 1994).