Worm Breeder's Gazette 13(2): 48 (February 1, 1994)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

A screen for proteins that interact with GLP-1 ankyrin repeats.

Henry Roehl, Judith Kimble

University of Wisconsin, Madison, WI 53706.

GLP-1 is a receptor that is involved in several cell fate decisions during development. We previously showed that a fragment from the intracellular region of GLP-1 ,which is predominantly made up of ankyrin( cdc-10 /SWI6)repeats, is capable of directing cell fate decisions(1). The ankyrin repeats of other proteins (eg 1KB and GABPß) mediate protein-protein interactions. Our hypothesis is that the GLP-1 ankyrin repeats control cell fate by binding one or more downstream, target proteins. We are attempting to identify proteins that interact with GLP-1 ankyrin repeats in vitro, and then investigate the role of the interacting proteins in vivo. We have screened several expression libraries using radioactively labeled GLP-1 ankyrin repeats as a probe. This procedure has yielded several strong positives with little background. We are now checking these positives with several controls to see if the interaction is specific to the GLP-1 ]ankyrin repeats. Anyone who is using or is interested in using this method to identify interacting proteins should feel free to email me at in%"hroehl@macc.wisc.edu".

(l) Roehl & Kimble, (1993) Nature 364: 632-635