Worm Breeder's Gazette 13(2): 14 (February 1, 1994)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

The C. elegans genome sequencing project: A progress report.

The C. elegans Genome Consortium, Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri, USA and Sanger Centre, Hinxton Hall, Cambridge, UK.

Figure 1

We present here a progress report for the C. elegans genome sequencing project. A detailed description of 2.181 Mb of finished, contiguous sequence near the center of chromosome III has recently been accepted (1). This sequence, bracketed by cosmid clones ZK112 and ZK757 ,includes the mapped genes egl-45 , lin-36 , unc-36 , unc-86 , mig-10 , unc-116 , ceh-16 ,dpy- l9 , sup-5 , unc-32 , lin-9 ,gst-l lin-12 , glp-1 , emb-9 , tbg-1 and ncc-1 .All of the finished sequence data for this region has been placed in acedb and the GenBank and EMBL databases. Since the completion of the 2.181 Mb region, we have made considerable progress on other large stretches of chromosome III (Figure 1). Several small regions not shown between F45H7 and T21C12 are contained only on YAC clones and must be rescued and subcloned before the entire sequence can be completed. Protein similarity data for a part of the region is presented in Table 1 (database hits from the 2.181 Mb sequence are not included in this list). The two groups have used different criteria for determining which cosmids were included in the list. Cambridge has included only finished cosmids and those cosmids which are contiguous but still have one or more problem areas. St. Louis has also included cosmids which have one or two gaps but which are otherwise in good shape. Also, Cambridge has indicated the position and type of similarity within each cosmid, while St. Louis has listed the name and blastx score for the strongest hits. For future submissions we hope to be more consistent; our experience here should help us decide where to set boundaries for future Gazette releases. Although the cosmids which contain database hits may not be complete, the Consortium will make preliminary sequence data available to the community with the caveat that it is preliminary and may still contain errors. Furthermore, we are willing to help locate genes for persons having a bit of sequence data (or to provide an estimated completion time for a particular cosmid). In addition to chromosome III, sequencing has begun on chromosome II. Here, we have started near the lin-5 gene, with the St. Louis group proceeding left from cosmid C06A8 and the Cambridge group proceeding right from cosmid T05A6 .For information on homologies, please contact LaDeana Hillier (lhillier@watson.wustl.edu) or Richard Durbin (rd@sanger.ac.uk). For information on sequencing plans or estimated completion times, please contact Richard Wilson (rwilson@watson.wustl.edu) or Alan Coulson (alan@sanger.ac.uk). All requests for cosmid clones should be addressed to Alan Coulson.

(1)R. Wilson et al. Nature, in press

Literature Cited:

1. R. Wilson et al. Nature, in press.

Figure 1