Worm Breeder's Gazette 12(1): 38 (September 1, 1991)

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

A second transporter gene downstream of unc-22

Chris Fields

Figure 1

Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD 20892, USA.

The unc-22 cosmid appears to contain a gene encoding a protein with significant similarity to a mammalian GABA transporter. An alignment of the predicted protein sequence with that of the rat GABA transporter (PIR accession # A35918 )is shown below. The predicted gene is on the same strand as unc-22 .Its initiator ATG is at position 46268 in the unc-22 sequence obtained by Guy Benian and colleagues, 4 kb downstream of the polyadenylation site of unc-22 ,and roughly 2 kb downstream of the start of the glucose-transporter gene predicted to be on the opposite strand from unc-22 (WBG, September 1990). We will attempt to amplify and sequence a cDNA from this gene using primers specific for the predicted exons.

[See Figure 1]