Worm Breeder's Gazette 11(3): 22

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Cloning, Mapping and Splicing of the Tropomyosin Gene, tmy-1, of Caenorhabditis elegans

Koken Sugimoto and Hiroaki Kagawa

Figure 1

Genomic restriction fragments expanding 6.3kb were cloned by using 
exon expression fragment as a probe and were sequenced.  EcoRI-5kb 
fragment hybridized to the YAC clones;Y68B7 and Y38D6 LGI (Alan 
Coulson) and not hybridized to any fragment from cosmid clones K05C4 
and F39B2.  Hair pin structure of the intron part of the genomic 
fragment might interfere packaging of DNA fragment into phage particle.
Following figure presents the genome structure and three different 
cDNA clones which was obtained from cDNA library (the last issue of 
WBG #11-2).  Comparison with tropomyosin genes of Drosophila suggests 
that two were muscle type and one was cytoplasmic type.  Missing 'head 
and tail ' of the genome will help to know how the gene expresses in 
the worm.
[See Figure 1]

Figure 1