Worm Breeder's Gazette 11(2): 21

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Accidental Cloning of a Ribosomal Protein Homologue Transspliced to SL2

Andrew Fire and Susan Harrison

Figure 1

In a PCR of cDNA using myo-3 (antisense) and SL2 (sense) primers (
SL2=splice leader 2; Huang and Hirsh WBG 10 #3 p79) we obtained a 
prominent band under moderate stringency cDNA synthesis conditions.  
When we cloned and sequenced this band we found no homology to myo-3 
but rather a sequence which encodes a ribosomal protein homologue.  
The initial SL2 oligo contained only the first 17 bases of the 22bp 
leader.  The presence of the other 5 nucleotides of SL2 in the cDNA 
clones confirms that SL2 was indeed present on the 5' end of the gene. 
No equivalent band was found when a similar SL1 oligo was used as 
primer.
[See Figure 1]

Figure 1