Worm Breeder's Gazette 11(1): 22

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Cloning mig-10

Jim Manser

Figure 1

Correlation of the genetic and physical maps has been exploited to 
clone the mig-10 III gene (see CSH '89 Abstracts and previous WBG's).  
Genetic mapping placed mig-10(ct41) between unc-86 and unc-116, 
corresponding to a relatively small interval of the physical map (see 
Figure).  A set of 5 cosmids from this interval was chosen and used in 
co-transformation rescue experiments with an unc-36 
41) strain.  One cosmid, F10E9, was found to rescue 
mutant phenotypes, placing mig-10 on the physical map.  Specifically, 
4 of 9 transgenic lines exhibiting unc-36 rescue also displayed mig-10 
rescue, and in two such lines mig-10 rescue was transmitted to 
subsequent generations.  To localize mig-10 further, several cosmids 
that overlap F10E9 are being tested for rescue, as are lambda 
subclones of F10E9.
[See Figure 1]

Figure 1