Worm Breeder's Gazette 10(3): 94
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We are attempting to identify all the essential genes in a large genomic region. A new set of 242 strains carrying 0.012M EMS induced lethal and sterile mutations on the 23 m.u. of LGV(left) that is balanced by the translocation eT1(III:V) are being analyzed. To facilitate this work, the break points of 31 deficiencies and 1 duplication have been mapped and used to subdivide LGV(left) into 36 zones. Prior to this study 59 essential genes had been identified on LGV(left) (Rosenbluth, Rogalski, Johnsen, Addison and Baillie Genet. Res., In Press). We have analyzed 95 of the new 242 strains with EMS induced lethal mutations. The results are: [See Figure 1] A Poisson calculation shows that their are a minimum of 97 essential genes on LGV(left). This implies that we have hit at most 81% of the essential genes on LGV(left).