Worm Breeder's Gazette 10(3): 9
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We have identified a new her-1 gain-of-function mutation, her-1( y101sd). y101 was isolated as a dominant Egl that segregated abnormal males. her-1(y101sd) seems to be a stronger gain-of-function mutation than the previously described her-1(n695sd) (Trent et al. Genetics 120:145-157), since at 20 C approximately 60% of y101 XX homozygotes develop as pseudomales or intersexes compared to approximately 20% for n695. We have used two criteria to assign the y101 mutation to the her-1 gene. First, y101 is tightly linked (<1 m. u.) to unc-42(e270)V, which maps 0.2 map units from her-1. Second, we have obtained three new recessive her-1(lf) alleles as revertants of the dominant Egl and Tra phenotypes of y101. (4000 EMS-mutagenized haploid genomes were examined in this revertant screen, which also yielded four extragenic suppressor mutations as well as two Fem mutations.)