Worm Breeder's Gazette 10(3): 54

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Some unc-31 Sequence

Roger Hoskins

The Sequence of a 4.5kb genomic clone that covers nine of ten 
rearrangements in unc-31 alleles is almost complete.  A necessarily 
tentative interpretation of the data is that the sequence includes the 
3' end of the unc-31 gene.  A good worm consensus splice acceptor 
sequence is followed by a 495bp ORF, with a polyadenlyation signal 
280bp further along.  No significant homologies were detected when the 
entire 4.5kb sequence was translated in all 6 frames and compared to 
the PIR protein sequence database using the Lipman and Pearson program 
There is a 17bp inverted repeat (GATGCGCGGAACCCAAA) separated by a 
90bp spacer at one end of the sequence.  Given that unc-31 is 
apparently a 'hot target' in mutator strains and that most 
rearrangements in mutant alleles of the gene, including five deletions,
map within the sequence, apparently at one end of the gene, the 
repeat may have significance.
I have confirmed that the lambda-phage CB#RH12 and CB#RH17 rescue 
unc-30(e191).  In addition, a third clone CB#RH11 rescues, but it 
seems not completely.  Transformed animals move much better than the 
mutant, but not as well as wild-type.  Very little transmission of the 
rescued phenotype to subsequent generations has been seen, so it is 
possible that the clone contains all of the unc-30 gene, but is less 
apt to form semi-stable extra-chromosomal arrays.  Two pUC subclones 
spanning the 8kb overlap region have so far failed to rescue.  I plan 
to try cotransformation experiments with unc-31 rescuing DNA to test 
these, and to begin sequencing.