Worm Breeder's Gazette 10(3): 45
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Complete nucleotide sequence data of paramyosin gene, unc-15 will appear in the EMBL/GenBank/DDBJ Nucleotide Sequence Databases under the accession number X08068. By the friendly information from St Louis, Larry Shriefer and Bob Waterston, a head piece of paramyosin was extended a little bit. Finally, the gene is composed of 10 exons encoding a protein of 866 amino acid with molecular mass of 99,890. The gene had no TATA-box and promoter like sequences at 5' upstream as other muscle genes of the worm. Manuscript entitled 'The paramyosin gene (unc-15) of Caenorhabditis r cloning, nucleotide sequence and models for thick filament assembly' collaboration with Andrew D. McLachlan, Sydney Brenner and Jonathan Karn was submitted to J. Mol. Biol. cDNA clone of tropomyosin gene was cloned from a cDNA library (48hr sample) which was constructed by Julie Ahringer and Judith Kimble by using a tropomyosin gene fragment of C. elegans as a probe. The fragment of probe was cloned by screening an exon expression plasmid library (1985 C. elegans meeting abstract) with anti-tropomyosin antibody. Cloned 1.6kb fragment encoded the 6th to 241th amino acid residue of tropomyosin. The amino acid sequence was very conserved. To clone a complete fragment of the gene of genomic lambdoid phage library (kindly supplied by Claudia Cummins and Phil Anderson) was screened by using 1.6kb of cDNA clone as a probe. Ten positive clones were processed and mapped on the cosmid library by Alan Coulson and John Sulston. Unfortunately, all clones was contained the identical sequence to other part of unrelated cDNA (Worm gazette, Vol. 10, No. 1) which located the upstream of the cDNA clone of tropomyosin. The junction sequence of the both fragment was as follows. We are not sure why tropomyosin gene was not contained in the genomic library. [See Figure 1] Tropomyosin gene will be cloned from a genomic library which will construct with the genomic restriction fragment of the correct size. Original gene of cloned fragment of other part leaves in question.