Worm Breeder's Gazette 10(3): 36

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Homeo Box Containing Genes in Ascaris lumbricoides and C. elegans

A. Spicher, D. Schaller, C. Wittmann, F. Muller and H. Tobler

Figure 1

The study of homeo box containing genes in nematodes has been 
approached by using the homeo boxes of the Drosophila genes fushi 
tarazu (ftz) and Antennapedia (Antp) as hybridization probes.  From a 
genomic library of A.  lumbricoides, several positive clones were 
isolated and one of them was further investigated.  Sequence analyses 
revealed the existence of a 180bp lone Ascaris homeo box (AHB-1), 
located within a short, 243bp lone open reading frame.  The AHB-1 
encodes an amino acid sequence which is 78% homologous to the 
Drosophila Antp homeo domain.
By using the Ascaris sequence AHB-1 as a probe, we succeeded in 
isolating two different homeo box containing clones from a C.  elegans 
genomic library, kindly provided by Drs. S.  Ward and K.  Bennett.  
The sequence of one of them (CELHO) was analyzed and was shown to 
encode a polypeptide which is 57% homologous to the Drosophila Antp 
homeo domain.  Neither the Ascaris nor the C.  elegans homeobox 
contains an intron.  Furthermore, the C.  elegans homeobox was used as 
probe for screening the Ahringerer cDNA library of eggs.  Three cDNA 
clones were picked.  We are currently investigating the pattern of 
expression of these genes during development.
[See Figure 1]

Figure 1