Worm Breeder's Gazette 10(3): 140
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Rose and Baillie (1979) characterized recombination in the hermaphrodite and found it varied with temperature and age. We have undertaken a similar study to characterize recombination in the male. To examine the effects of age, dpy-5(e61) 50)/++ males were mated to 5 homozygous hermaphrodites every 12 hours for 108 hours and the outcross progeny was scored. A recombination frequency of 1.14%(95%C.I.1.11-1.16) was observed for this interval, a 33% decrease compared to the hermaphrodite control. This frequency also showed variation with age. Intervals outside the cluster on LGI are also being studied in order to examine the possibility of regional effects. We were also interested in examining recombination in hermaphrodite sperm. Brenner (1974) used sex-linked markers in the egg to determine recombination frequencies in both germlines, which he found to be approximately the same. Males of the genotype dpy18 hT2/dpy-5 unc-13 were mated to dpy-5 hermaphrodites every 24 hours over a 96 hour period. The translocation acts as a cross-over suppressor in the male and scoring the male progeny gives a direct measure of the recombination frequency in the oocytes. This number can then be used to calculate the frequency in the hermaphrodite sperm. Initial experiments indicate the recombination frequency in the two germlines is not the same in the autosomal interval tested. Further experiments will examine the effects of rec-1, a general recombination enhancer, on recombination in the male.