Worm Breeder's Gazette 10(3): 139

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Refining the Map of the Far Right End of the X Chromosome

Anne M. Villeneuve and Barbara J. Meyer

Figure 1

For a variety of reasons, we have been doing a lot of mapping in the 
lin-15 -10 interval on the right arm of X.  This 
mapping has identified errors in the previously published map 
positions of several genes.  Since it will be several months before 
the 1989 issue of the map is ready, and since we have already received 
four separate requests for our revised map from people that are 
cloning genes in the region, we felt it would be expedient to include 
it here.
The genes shown on the map have all been ordered by three-factor 
crosses (the data will be available in the CGC map data update).  Only 
genes that were used in these mapping experiments are shown.  osm-1 is 
placed above the line because it has been mapped to the left of sup-10 
but has not been ordered with respect to mec-4 or let-2.  The distance 
of 0.8 m. u.  between lin-15 and sup-10 is based on 2-factor data; 
other distances are interpolated from 3-factor data.  The hole in 
mnDp8 is inferred from the fact that although let-2 clearly maps to 
the left of mec-4, nts lin-15, 
osm-1, and mec-4, but NOT let-2 or sup-10.TCMEC4A 
and TCMEC4B are Tc1 polymorphisms mapped by Monica Driscoll.  By three-
factor mapping, TCMEC4A maps between let-2 and mec-4 and TCMEC4B maps 
either very close to or to the right of mec-4.  Subsequently, TCMEC4A, 
sup-10, and TCMEC4B were joined on a single contig.  By combining 
genetic and physical data, we can thus orient the contig with respect 
to the genetic map and place TCMEC4B to the right of sup-10.[See 
Figure 1]

Figure 1