Worm Breeder's Gazette 10(2): 147
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We have isolated an allele of glp-1 that suppresses recombination on the third chromosome between at least dpy-1 and dpy-18 9) was generated by gamma-ray mutagenesis and is linked to e1259. In the strain dpy-19(e1259)glp-1(q339)/unc-32(e189) dpy-18(e164) we observed that the expected unc-32 and dpy-18 recombinants were absent. To investigate this phenomenom further we looked for Dpy and Unc recombinants in strains heterozygous for e1259q339 and a series of Dpy on Chromosome III: dpy-1(e1)unc-93(e1500), 500)dpy-17(e164), 64)unc-32(e189), 89)dpy-18(e364) and dpy-18(e364)unc-64(e246). For each strain six broods were scored The only recombinant found was a single dpy-18 recombinant in the strain dpy-19(e1259)glp-1(q339)/dpy-18(e364)unc-64(e246) We think that the suppression of recombination by the e1259q339 chromosome is specific to Chromosome III rather than a generalized suppression of recombination because dpy-19(e1259)glp-1(q339)/+ + III; unc-60(e723) dpy-11(e224)/+ + V animals segregate both unc-60 and dpy-11 recombinants at the expected frequency. The e1259q339 chromosome contains a DNA rearrangement with a breakpoint in the glp-1 gene (Austin and Kimble this issue) This rearrangment may be the cause of the suppression of recombination Although we have not yet determined the nature of this rearrangment, no dead eggs are laid by e1259 q339 heterozygotes, suggesting that it is not a reciprocal translocation