Worm Breeder's Gazette 10(2): 139
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We have investigated the possibility of using RFLD's (Restriction Fragment Length Differences) of a mtDNA fragment to distinguish the various C. elegans strains. Ann Rose's lab cloned the 5kb EcoRI restriction fragment (pCeh109) of the mitochondria DNA. Total DNA from N2, BO, PA-2, GA-1, and N2(DR) was isolated, restricted, fractionated on an agarose gel, and transferred to nitrocellulose. The filter was then probed using [32p] labelled pCeh109. The restriction enzymes we used were AccI, AluI, AvaII, DdeI, DraI, HaeIII, HincII, HinfII, and RsaI. Six of the nine enzymes used showed an RFLD that easily distinguished Pa2 from the others, and two showed a possible difference between N2 and BO. Since PA-2 shows an RLFD with so many of the restriction enzymes, it is possible that the mtDNA of this strain has some sort of rearrangement. The nature of these RLFD's and the ones between N2 and BO are currently being investigated.