Worm Breeder's Gazette 10(2): 138
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
As part of our ongoing genetic characterization of essential genes in the unc-22 (IV) region, we have isolated 7 new unc-22 deficiencies. The reasons for this are threefold: 1) to order genes in the region more precisely, 2) to make further mapping of EMS induced lethals less laborious and 3) to have a larger set of deficiency breakpoints for landmarks on the physical map. Our screen involved the exposure of unc-31(e169) hermaphrodites to 0. 1% formaldehyde for 4 hr. and screening in the F1 generation for twitchers in a 1% nicotine solution, indicating a mutation in unc-22. The progeny of the selected F1's were then screened for the absence of Unc-22 als, indicating the presence of a possible deficiency. About 500,000 F1 chromosomes were screened to get 7 deficiencies (sDf60 to 65 and sDf67). Their positions on the map are indicated below. The right breakpoint of sDf60 lies off this map between unc-31 and unc-26.[See Figure 1]