Worm Breeder's Gazette 10(2): 120

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Partial Sequence of the unc-6 Gene

N. Ishii, B. Stern, E. Hedgecock and J. Culotti

Figure 1

In the previous issue (WBG 10(1) p.35), we reported cloning a small 
DNA fragment containing the Tc1 insertion which caused the unc-6 (
rh1035) mutation.  Since then, we have isolated an 11.5 kb EcoRI 
fragment including this region from N2 DNA (NJ#14, EMBL4 vector) Alan 
Coulson has kindly positioned this fragment on the MRC genome map.  We 
have now sequenced both the original 1.25 kb XbaI fragment of unc-6(
rh1035) DNA (plasmid NJ#6) and a 2.7 kb HindIII fragment of N2 DNA 
subcloned from phage NJ#14.  The 2.7 kb HindIII fragment has five 
exons which encode a hypothetical protein fragment of 360 residues.  
This fragment is not closely related to any protein in the current PIR 
database The last exon in the 2.7 kb HindIII fragment is followed by 
an intron which continues beyond the fragment.  The first exon is 
preceded by a non-coding region which may be part of an intron or the 
unc-6 promoter region.  In fact, a possible 21 residue signal sequence 
with an initiator methionine occurs in the first exon shortly upstream 
of the Tc1::rh1035 insertion site.  TATA and CCAAT motifs occur about 
90 and 120 bp upstream of the proposed initiator codon.  The 
decanucleotide sequence TTTGCCTCTC is tandemly repeated about 200 bp 
upstream of this codon.
[See Figure 1]

Figure 1