Worm Breeder's Gazette 10(1): 81
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Ferguson and Horvitz (Genetics 110:17-72, 1985) identified nT1(IV;V) as a crossover suppressor of LGIV(right) (unc-17 to dpy-4) and LGV( left) (unc-60 to dpy-11). They also found that nT1 pseudolinked these two regions. nT1 is associated with a recessive vulvaless phenotype. We have screened for lethal mutations in strains heterozygous for nT1 [see WBG 9(2):82 and 9(3):31]. As a result, we wanted to know more precisely the extent of nT1 crossover suppression. We thought it would be useful for others working on LGIV and LGV to know our results. a b/nT1(IV);+/nT1(V) and +/nT1(IV);a b/nT1(V) individuals were constructed and their progeny were scored for recombinants. The data is listed in the table below. In summary, nT1 effectively balances 21 m.u. from lin-1 (e1777 supplied by Horvitz lab) to dpy-4 on LGIV and 25 m.u. from unc-60 to unc-76 on LGV. The unc-17 and unc-22 recombinant chromosomes from a b/nT1 individuals (where 'b' is dpy-4) did not carry nT1. Therefore, nT1 does not balance LGIV in the region just to the left of dpy-4.[See Figure 1]