Worm Breeder's Gazette 10(1): 42
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Protein kinase C (PKC) is considered to play an important role in the signal transduction of the eukaryotic system and proposed to be a primary target of TPA. As a part of identifying PKC-like genes to understand the structural and functional relationship between tpa-1 and PKC-like genes, we carried out Southern hybridization analysis using rabbit PKC cDNA(alpha, and gamma forms kindly provided by Dr. Suzuki/Dr. Ohno, Tokyo Metropolitan Institute of Medical Science) as a probe. Five major bands of 6.0, 4.1, 3.2, 3.0, and 1.2kb and several minor bands were detected in HindIII -digested N2 DNA. Also, to clone PKC-like genes we have screened both a cDNA library (a gift from Dr. Kimble/ Dr. Ahringer) and a genomic library with the same rabbit PKC DNA. We have obtained 2 and 7 clones, respectively. We are testing homology between these cloned DNA's and putative tpa-1 cDNA as well as a rather long stretch of genomic DNA covering the 2. 4kb fragment associated with Tc1-induced TPA resistance.