Worm Breeder's Gazette 10(1): 17

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Collagens, Cuticles and Chromosome V

K. Edwards

Figure 1

On the right side of the cluster on Chromosome V are several genes 
which, when mutated, cause defective cuticles.  These genes include 
vab-8, rol-4,  
cuticles are comprised predominantly of collagens, I wanted to 
determine whether any of these genes do in fact encode a collagen.  
Using radioactivity-labeled pcol-2, I probed restriction digests of 
overlapping cosmids contained within the myo-3 contig which had been 
mapped to this region.  (See maps below).  Two of these cosmids, 
C12A12 and ZC348, cross-hybridized to pcol-2 under low stringency 
conditions.  Interestingly, the HindIII fragment of ZC348 had the same 
molecular weight (3.5 Kbp) as did the HindIII fragment of pcol-1.  As 
a further test for identity, I probed double digests of both ZC348 and 
pcol-1 (HindIII/BamHI and Hind III/HinFI) with labeled pcol-1 under 
high stringency conditions.  Identical restriction fragments were seen 
for both clones.  The localization of col-1 to this region is a 
tremendous aid to our study of cuticle-defective mutants: col-1 has 
been sequenced (Kramer et al., 1982, Cell 30, 599) and its pattern of 
developmental expression has been determined (Cox & Hirsh, 1985, Mol.  
Cell.  Biol.  5, 363).  We are now using the col-1 gene as a probe to 
look for RFLPs in mutant DNAs.  To study the function of the col-1 
gene product we plan to isolate specific antibodies which can be used 
for biochemical and morphological analyses of wild-type and mutant 
[See figure 

Figure 1