Worm Breeder's Gazette 10(1): 16
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
The unc-30 gene has been found to lie on the physical map, in a contig previously shown by J. Yuan to span the ced-3 locus(CSH 1987). unc-30 recombinants were picked from the progeny of unc-31(e928)unc- 30(e191)/BO parents and used to map BO/N2 DNA polymorphisms found in the ced-3 contig. The data indicate that unc-30 is surprisingly close to ced-3.Two polymorphisms have been mapped. They have been designated eP56 and eP57, and are recognized by hybridization with the cosmids ZK987 and C43A2, respectively. They map as follows: unc- 31eP56ePSunc-30. A right-hand limit on the position of unc- 30 has been established by Yuan. She has mapped a BO-specific Tc1 to the right of ced-3 in the cosmid MMMC1. The physical distance between unc-30 and ced-3 must therefore be less than 120kb of DNA; the genetic distance between them is 0.3-0.5%. It should now be possible to clone the unc-30 gene. Attempts toward this end have been hampered by the potpourri of repeat sequences in cosmids to the right of eP57. Several DNA polymorphisms have been found in EMS, [32P], and spontaneous BO unc-30 alleles.