Worm Breeder's Gazette 10(1): 12
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clones analyzed = 17545 clones in contigs = 17092 contigs = 680 % genome in contigs = 89. 5 mean contig size (Kb) = 105 contigs >200Kb = 79 unattached clones = 453 maximum % genome in unattached clones = 19. 3 As we indicated at Cold Spring Harbor, Bob Waterston has constructed a C. in a yeast artificial chromosome (YAC) vector. The mean insert size is 100-150 Kb. 2100 YAC's have been isolated and gridded and we have started using them as probes to make joins in the cosmid map. In addition, a limited number of replicas of the grid are available from the Medical Research Council (MRC). First reports are good, in that a number of people have found positives with their probes. We are not entirely clear how best to use the YAC's thus identified; however, since many individual walking efforts, as well as generalized closure of the map, are going to revolve around this grid for a time, the more information that we can collect about it the better. We would, therefore, like to send replicas to those labs that are interested in probing them and ask in return to be given the hybridization data that you collect. YAC's of interest to you are available from either St. Louis or the MRC. The part of the current map marked by cloned genes or polymorphisms is summarized in the following table. Contigs on each chromosome are ordered in three blocks: (1) unknown position; (2) known physical position (by in situ hybridization); (3) known genetic but unknown physical position. Because of the distortion of the genetic map relative to the physical map, there is no systematic way to unite (2) and (3); but as linkage proceeds, everything will move into (2).