Worm Breeder's Gazette 10(1): 12

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Genome Map

A. Coulson, J. Sulston, D. Albertson, R. Fishpool, R. Waterston and H. Ameer

clones analyzed = 
clones in contigs = 
contigs = 
% genome in contigs = 89.
mean contig size (Kb) = 
contigs >200Kb = 
unattached clones = 
maximum % genome in unattached clones = 19.
As we indicated at Cold Spring Harbor, Bob Waterston has constructed 
a C. in a yeast artificial chromosome (YAC) 
vector.  The mean insert size is 100-150 Kb.  2100 YAC's have been 
isolated and gridded and we have started using them as probes to make 
joins in the cosmid map.  In addition, a limited number of replicas of 
the grid are available from the Medical Research Council (MRC).  First 
reports are good, in that a number of people have found positives with 
their probes.  We are not entirely clear how best to use the YAC's 
thus identified; however, since many individual walking efforts, as 
well as generalized closure of the map, are going to revolve around 
this grid for a time, the more information that we can collect about 
it the better.  We would, therefore, like to send replicas to those 
labs that are interested in probing them and ask in return to be given 
the hybridization data that you collect.  YAC's of interest to you are 
available from either St. Louis or the MRC.
The part of the current map marked by cloned genes or polymorphisms 
is summarized in the following table.  Contigs on each chromosome are 
ordered in three blocks: (1)  unknown position; (2) known physical 
position (by in situ hybridization); (3) known genetic but unknown 
physical position.  Because of the distortion of the genetic map 
relative to the physical map, there is no systematic way to unite (2) 
and (3); but as linkage proceeds, everything will move into (2).