Worm Breeder's Gazette 1(2): 9a

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Title unknown.

Authors unknown.

Adults, larvae and eggs were washed off a large Petri plate with M9 
salts and collected by centrifugation.  Bacteria and worms were 
dissolved by incubating in a solution of 1-2% NaOCl and 0.5 N NaOH for 
10 mins. at room temperature.  The remaining purified eggs were 
collected by centrifugation (IEC clinical centrifuge, setting 3, 2 
mins.) and washed 3 times with M9 salts.  Ten thousand to 50,000 eggs 
per large Petri plate were obtained.  Over 90% of the cleaned eggs 
hatched.  Eggs can be purified further by centrifugation in a linear 5-
30% w/v sucrose gradient in the clinical centrifuge for 10 mins.  at 
setting 3.  Eggs reach equilibrium quickly and form a band in the 
middle of the gradient.
When eggs of the temperature sensitive mutant, tsB73, are raised to 
25 C, those eggs that are 7 hours or more prior to hatching develop 
into worms with a pear shape.  Eggs that are passed this critical time 
develop into worms with wild type morphology.  Purified tsB73 eggs 
were centrifuged to equilibrium in a 15-20% sucrose gradient in SW27 
tubes.  The gradient was collected and the eggs were plated at 25 C.  
The eggs of lighter buoyant density hatched first and 90% had wild 
type morphology.  The eggs on the heavier side hatched later and were 
enriched for the pear morphology.  Therefore, early embryos are 
heavier than later ones.  This might be a useful method for staging