Worm Breeder's Gazette 1(2): 5a

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Title unknown.

Authors unknown.

Nematodes can be grown conveniently in gram quantities on plates.  
Using the Cambridge bacterial strain, NA22 and increasing the amount 
of Bactopeptone in NGM media from 0.25% to 2% in 0.25 steps results in 
an approximately linear increase in yield on 8.5 cm diameter petri 
dishes.  The settled volumes are 0.03 ml to 0.22 ml of worms per plate 
for 0.25% and 2%, respectively.  N2, E675, and E190 have been grown 
this way.
l) I can't guarantee that a single nematode would behave in this 
manner.  All of my work has involved washing nematodes off NGM plates 
with M9 buffer, letting the nematodes settle, and then pipetting the 
nematodes onto enriched peptone plates at no more than a 10x dilution.
2) Although 2% Bactopeptone plates may not be the limit for 
linearity of nematode growth, a medium can be too rich.  For example, 
3xD plates cause nematodes to go into dauer or die.