Worm Breeder's Gazette 1(2): 25b
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
We have studied the variation in recombination between two markers, dpy 561 and unc 1351 with respect to temperature and oocyte age. Our results show that recombination frequency changes constantly during the production of gametes, both in the male and hermaphrodite. There is no particular stage at which precise recombination analysis can be done. It is therefore suggested that all recombination analysis be done by sampling the total supply of gametes of a P-O, hence, taking an average recombination value over the reproductive cycle. Temperature also shows a marked effect on recombination. Recombination analysis, therefore, must be done at a defined temperature. We suggest that 25 C is the most convenient temperature for these studies. A full account of this work will be presented in a forthcoming publication.