Worm Breeder's Gazette 1(2): 11
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
Some French strains of C. elegans exhibit a zygote-defective phenotype at 25 C. Crosses of English (N2) males with French ( Bergerac) hermaphrodites (obtained from Dr. R. Stokstad, Univ. of Calif., Berkeley, through Carl Johnson) yield progeny that are fertile at 25 C, although some F2 segregants show reduced fertility. When A and B tests for maternal effect (see preceding report) were carried out using F1 hermaphrodites from such a cross and wild type Bergerac hermaphrodites, respectively, the French strain scored M,M. Therefore, the ts lesion in this strain is recessive, and the gene(s) that it defines must be expressed in the parent for zygote survival. Linkage of the ts allele(s) was tested by crossing English males heterozygous for known dpy markers with French hermaphrodites and scoring F2 dpy progeny for the ts phenotype. The ts character is linked to dpy-10 in linkage group II. The N2 ts zyg mutant B1 characterized by Vanderslice and Hirsh exhibits the same maternal effects, and linkage as the Bergerac strain, and displays a lethal phenotype at 25 C very similar to that of the French strain studied by Nigon. However, the two alleles complement; a tsB1/ts Bergerac heterozygote was constructed and found to be fertile.