Worm Breeder's Gazette 1(1): 21b
These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.
I too have been examining ts mutants. I developed an enrichment method for their isolation. Roughly synchronized F2 populations are grown at 21 C. Slow developers are separated from normal developers by differential settling. Of these slow developers up to 20% prove to be temperature sensitive mutants. Seventy-three such mutants were isolated and partially characterized. The major class (28) prove to be sensitive to high temperature at all stages of the life cycle (poop- outs) and are presumed to affect general cell metabolism. The rest could be classed as zyg (a block in embryogenesis), gon (steriles-- most appear defective in oogenesis, a few seem specifically defective in gonad development),zyg-gon (mutants with both of the above phenotypes) and sp (spermatogenesis defectives). Of my zyg mutants most are zyg-gon. Most show a maternal effect. Reconstruction experiments show that the enrichment technique probably works mainly for the isolation of poop-outs. Many mutants were isolated in a HIM-2 background. Unfortunately, HIM-2 (E1065) appears to be a temperature sensitive general non-disjunction mutant and produces 5% males and about 25% sterile eggs at 25 C.