Worm Breeder's Gazette 1(1): 14

These abstracts should not be cited in bibliographies. Material contained herein should be treated as personal communication and should be cited as such only with the consent of the author.

Progress Report

T. Cole, U. Deppe, G. von Ehrenstein, C. Krieg, E. Schierenberg, D. Schmitt, B

This group works on C.  elegans for about two years.  Our main 
interest is the development of this organism.
We are serial sectioning eggs in various cleavage stages to trace 
the cell lineage.  Completed so far: 1 egg, 2 two-cell stages, 1 four-
cell stage, 1 bretzel-stage.  An assembly line is being organized and 
hopefully the turnout will speed up.  Nomarski studies on cleaving 
eggs supplement the EM studies.  A detailed study of the 
ultrastructure of the cleavage furrow and the formation of cell 
membranes at early cleavage stages has been initiated.  We are 
reconstructing 3-D pictures using a PDP 11/45 computer with a Vector 
General Graphics Display.  Work has been started to accumulate a set 
of cleavage-deficient mutants for morphological analysis.  In addition 
some preliminary experiments show that eggs can be opened by treatment 
with a variety of proteolytic enzymes.  In this way suspensions of 
embryonal cells can be obtained.
Ruth Pertel is spending 4 months as a guest in the lab to teach us 
axenic cultivation and replica plating.  She also is interested in 
defining differences between the two strains of C.  elegans Bristol N2 
and NIH that have been kept separate for a number of years.  Jerzy 
Nowak from Poland worked 4 months on proteolytic enzymes in cleavage 
stages.  He also started using the O'Farrell technique (J.B.C.  250, 
4007-4021, 1975) for high resolution two-dimensional electrophoresis 
of proteins from eggs and worms.  Randy Cassada will join the group as 
a staff member starting December 1975.