CGC Bibliography Paper 5765

SAS-4 is essential for centrosome duplication in C. elegans and is recruited to daughter centrioles once per cell

Leidel S, Gonczy P

Medline:
12636923
Citation:
Developmental Cell 4: 431-439 2003
Type:
ARTICLE
Genes:
plk-1 sas-4 zyg-1
Abstract:
The mechanisms governing centrosome duplication remain poorly understood. We identified a gene called sas-4 that is essential for this process in C. elegans. SAS-4 encodes a predicted coiled-coil protein that localizes to a tiny dot in the center of centrosomes throughout the cell cycle. FRAP experiments with GFP-SAS-4 transgenic embryos reveal that SAS-4 is recruited to the centrosome once per cell cycle, at the time of organelle duplication. Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle.