CGC Bibliography Paper 5672

Functional posttranslational proteomics approach to study the role of N-glycans in the development of Caenorhabditis elegans.

Schachter H, Chen S, Zhang W, Spence AM, Zhu S, Callahan JW, Mahuran DJ, Fan X, Bagshaw RD, She YM, Rosa JC, Reinhold VN

Medline:

12655770

Citation:

Biochemical Society Symposium 69: 1-21 2002

Type:

REVIEW

Genes:

gly-12 gly-13 gly-14

Abstract:

Glycosylation is one of the most common post-translational protein modifications. Carbohydrate-mediated interactions between cells and their environment are important in differentiation, embryogenesis, inflammation, cancer and metastasis and other processes. Humans and mice with mutations that prevent normal N-glycosylation show multi-systemic defects in embryogenesis, thereby proving that these molecules are essential for normal development; however, a large number of proteins undergo defective glycosylation in these human and mouse mutants, and it is therefore difficult to determine the precise molecular roles of specific N-glycans on individual proteins. We describe here a 'functional post-translational proteomics' approach that is designed to determine the role of N-glycans on individual glycoproteins in the development of Caenorhabditis elegans.