CGC Bibliography Paper 5593

No reduction of energy metabolism in Clk mutants.

Braeckman BP, Houthoofd K, Brys K, Lenaerts I, De Vreese A, Van Eygen S, Raes H, Vanfleteren JR

Medline:

12425951

Citation:

Mechanisms of Ageing & Development 123: 1447-1456 2002

Type:

ARTICLE

Genes:

clk-1 clk-2 clk-3 gro-1

Abstract:

Mutation in any of the four clock genes (clk-1, clk-2, clk-3, gro-1) causes an average slowing down of many temporal processes, and an increase of mean life span. The latter effect has been linked to the slow phenotype, and it has been the ageing process. To test this model we measured several parameters describing metabolic output in wild type worms and all four Clk mutants. We found no gross changes in metabolic output, as assessed from oxygen consumption and heat production rates, lucigenin-mediated light production capacity, ATP content, and lipofuscin autofluorescence. Catalase and superoxide dismutase (SOD) were variably altered, but not cooperatively, as would be expected to enhance reactive oxygen species (ROX) scavenging activity. Thus we conclude that the prolonged life span of Clk mutant cannot be attributed to reduced metabolic rate or an increased activity of the major antioxidant enzymes catalase and SOD.