CGC Bibliography Paper 5565

Centrosome maturation and mitotic spindle assembly in C. elegans require SPD-5, a protein with multiple coiled-coil domains.

Hamill DR, Severson AF, Carter JC, Bowerman B

Medline:

12431374

Citation:

Developmental Cell 3: 673-684 2002

Type:

ARTICLE

Genes:

air-1 dhc-1 let-354 let-361 let-363 let-364 let-371 spd-2 spd-5 zyg-1 zyg-8 zyg-9 hDf28

Abstract:

The maternally expressed C. elegans gene spd-5 encodes a centrosomal protein with multiple coiled-coil domains. During mitosis in mutants with reduced levels of SPD-5, microtubules assemble but radiate from condensed chromosomes without forming a spindle, and mitosis fails. SPD-5 is required for the centrosomal localization of gamma-tubulin, XMAP-215, and Aurora A kinase family members, but SPD-5 accumulates at centrosomes in mutants lacking these proteins. Furthermore, SPD-5 interacts genetically with a dynein heavy chain. We propose that SPD-5, along with dynein, is required for centrosome maturation and mitotic spindle assembly.