CGC Bibliography Paper 5530
Nematode gene sequences, update for June 2002.
McCarter JP,
Clifton SW,
Bird DM,
Waterston RH
- Medline:
-
- Citation:
- Journal of Nematology 34: 71-74 2002
- Type:
- ARTICLE
- Genes:
-
- Abstract:
- High-throughput sequencing is revolutionizing molecular nematology by providing the sequences of thousands of genes never before characterized. The most rapid and cost-effective route to gene discovery for nematode genomes is the generation of expressed sequence tags (ESTs), single pass reads from random cDNA library clones that provide 300 to 600 nucleotides of sequence form a gene. Projects are currently under way at Washington University's Genome Sequencing Center that will generate 235,000 5'ESTs from approximately 25 nematode species by 2003 (119,448 to date). Additionally, the Sanger Institute and Edinburgh University are producing 80,000 ESTs from seven species (10,772 to date). New sequences are immediately submitted to the dbEST (database of expressed sequence tags) division of GenBank and are also available from a number of parasite-specialized Web sites (Table 1). Strategies for using ESTs, as well as discussions of the strengths and weaknesses of EST data, are available from reviews (Blaxter et al., 1999; Marra et al., 1998; McCarter et al., 200a);