CGC Bibliography Paper 5474

Ageing is reversed, and metabolism is reset to young levels in recovering dauer larvae of C. elegans.

Houthoofd K, Braeckman BP, Lenaerts I, Brys K, DeVreese A, Van Eygen S, Vanfleteren JR

Medline:

12213552

Citation:

Experimental Gerontology 37: 1015-1021 2002

Type:

ARTICLE

Genes:

Abstract:

The nematode Caenorhabditis elegans responds to unfavourable environmental conditions by arresting development and entering diapause as a dauer larva. Dauers can survive several times the normal life span and the duration of the dauer state has no effect on postdauer life span. This led to the suggestion that dauers are non-ageing, and that dauers eventually perish as the consequence of depletion of stored nutrients. We have investigated physiological changes associated with long-term diapause survival, and found that dauer larvae slowly develop senescence-like symptoms, including decrease of metabolic capacity, aconitase enzyme activity, and ATP stores, and increase of lipofuscin- and oxidised flavin-specific fluorescence. However, these changes are reversed when the dauers recover. Thus senescence can occur before attainment of reproductive maturity, and furthermore, is reversible. Other life processes, including respiration rate and heat output, remain unaltered over four weeks of diapause at 24 degreesC. Possible determinants of the enhanced life maintenance include increased resistance to oxidative stress provided by enhanced superoxide dismutase and catalase activities, and